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You receive a blood sample for HIV testing. What type of technique do you use?


Imagine you are a lab tech in a major hospital. You receive a blood sample for HIV testing. What type of technique would you use and what specifically are you looking for ? In other words are you looking for the presence of live virus, viral infected cells, antibodies etc? What,if anything, would you do differently than one of those send away HIV kits ? This may require a bit of digging on your part but its pretty interesting just the same. When you are HIV positive what exactly are you positive for ? Can you see a virus ? There is a specific technique which is used to determine whether a sample is positive or negative鈥?

...I don't think you should be a doctor.

I'll help you what I know. There are techniques that you use stains to determine if the virus is found in the blood. However, HIV is very naughty virus and it can stay dormant for up to 10 years. So, I would separate some of the blood, do a polymerase chain reaction (PCR), and the rest or the blood would be used for other more direct testing. With the polymerase chain reaction you can multiply the copies of DNA strands. Take this DNA and run a gel electrophoresis on it. Imprint the DNA sequence in a film paper and see if a pattern of the virus is seen in the DNA sequence.

the best strategy is to use:
1. BIOTEST HIV ELISA test to detect Ab
if it is positive u must do the secound step...
2.the confirmation test of western blot of GENELABS to confirm

If u patient is in high clinial risk it is better to do blot at first u know that Ab will be appeared in blood after at least 20 days.
Dr. Captain B

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