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Hiv Test?????????

despite the increased number of HIV tests, there is still no manufacturer that claims their test can be used to diagnose
infection with HIV. All of the RNA based tests for viral load and genotyping clearly state they are 'NOT intended for use in diagnosing HIV infection.'

Yes, and they react positive to nearly 100 unrelated conditions......

Factors Known to Cause
False Positive HIV Antibody Test Results

1.Anti-carbohydrate antibodies 52,19,13
2.Naturally-occurring antibodies 5,19
3.Passive immunization: receipt of gamma globulin or immune (as prophylaxis against infection which contains antibodies) 18, 26, 60, 4,
22, 42, 43, 13
4.Leprosy 2, 25
5.Tuberculosis 25
6.Mycobacterium avium 25
7.Systemic lupus erythematosus 15, 23
8.Renal (kidney) failure 48, 23, 13
9.Hemodialysis/renal failure 56, 16, 41, 10, 49
10.Alpha interferon therapy in hemodialysis patients 54
11.Flu 36
12.Flu vaccination 30, 11, 3, 20, 13, 43
13.Herpes simplex I 27
14.Herpes simplex II 11
15.Upper respiratory tract infection (cold or flu) 11
16.Recent viral infection or exposure to viral vaccines 11
17.Pregnancy in multiparous women 58, 53, 13, 43, 36
18.Malaria 6, 12
19.High levels of circulating immune complexes 6, 33
20.Hypergammaglobulinemia (high levels of antibodies) 40, 33
21.False positives on other tests, including RPR (rapid plasma
reagent) test for syphilis 17, 48, 33, 10, 49
22.Rheumatoid arthritis 36
23.Hepatitis B vaccination 28, 21, 40, 43
24.Tetanus vaccination 40
25.Organ transplantation 1, 36
26.Renal transplantation 35, 9, 48, 13, 56
27.Anti-lymphocyte antibodies 56, 31
28.Anti-collagen antibodies (found in gay men, haemophiliacs, Africans of both sexes and people with leprosy) 31
29.Serum-positive for rheumatoid factor, antinuclear antibody (both found in rheumatoid arthritis and other autoantibodies) 14, 62, 53
30.Autoimmune diseases 44, 29, 1O, 40, 49, 43
31.Systemic lupus erythematosus, scleroderma, connective tissue disease, dermatomyositis Acute viral infections, DNA viral infections 59,
48, 43, 53, 40, 13
32.Malignant neoplasms (cancers) 40
33.Alcoholic hepatitis/alcoholic liver disease 32, 48, 40, 10, 13, 49, 43, 53
34.Primary sclerosing cholangitis 48, 53
35.Hepatitis 54
36."Sticky" blood (in Africans) 38, 34, 40
37.Antibodies with a high affinity for polystyrene (used in the test kits) 62, 40, 3
38.Blood transfusions, multiple blood transfusions 63, 36, 13, 49, 43, 41
39.Multiple myeloma 10, 43, 53
40.HLA antibodies (to Class I and II leukocyte antigens) 7, 46, 63, 48, 10, 13, 49, 43, 53
41.Anti-smooth muscle antibody 48
42.Anti-parietal cell antibody 48
43.Anti-hepatitis A IgM (antibody) 48
44.Anti-Hbc IgM 48
45.Administration of human immunoglobulin preparations pooled before 1985 10
46.Haemophilia 10, 49
47.Haematologic malignant disorders/lymphoma 43, 53, 9, 48, 13
48.Primary biliary cirrhosis 43, 53, 13, 48
49.Stevens-Johnson syndrome 9, 48, 13
50.Q-fever with associated hepatitis 61
51.Heat-treated specimens 51, 57, 24, 49, 48
52.Lipemic serum (blood with high levels of fat or lipids) 49
53.Haemolyzed serum (blood where haemoglobin is separated from red cells) 49
54.Hyperbilirubinemia 10, 13
55.Globulins produced during polyclonal gammopathies (which are seen in AIDS risk groups) 10, 13, 48 cross-reactions 10
57.Normal human ribonucleoproteins 48, 13
58.Other retroviruses 8, 55, 14, 48, 13
59.Anti-mitochondrial antibodies 48, 13
60.Anti-nuclear antibodies 48, 13, 53
61.Anti-microsomal antibodies 34
62.T-cell leukocyte antigen antibodies 48, 13
63.Proteins on the filter paper 13
64.Epstein-Barr virus 37
65.Visceral leishmaniasis 45
66.Receptive anal sex 39, 64

Christine Johnson, a researcher and author, compiled this list of conditions documented in the scientific literature to cause positives on HIV tests, and provides references for each condition.

Christine notes:

"Just because something is on this list doesn't mean that it will definitely, or even probably, cause a false-positive. It depends on what antibodies the individual carries as well as the characteristics of each particular test kit.

For instance, some, but not all people who have had blood transfusions, prior pregnancies or an organ transplant will make HLA antibodies. And some, but not all test kits (both ELISA and Western blot) will be contaminated with HLA antigens to which these antibodies can react. Only if these two conditions coincide might you get a false-positive due to HLA cross-reactivity.

There are conditions that are more likely than others to cause false-positives. And there are some conditions that we aren't aware of yet which may be documented in the future to cause false-positives. Some of the factors on the list have been documented only for ELISA, while some have been documented for both ELISA and Western blot (WB) tests.

People may be eager to argue that if a factor is only known to cause false-positives on ELISA, this problem won't be carried over to the WB. But remember, a WB is positive by virtue of accumulating enough individual positive bands to add up to the total required by whatever criteria is used to interpret it 39. So the more exposure a person has had to foreign antigens, proteins and infectious agents, the more various antibodies he or she will have in their system, and the more likely it is
that there will be several cross-reacting antibodies, enough to make the WB positive.

It is to be noted that all AIDS risk groups (and Africans as well), but not the general US or Western European population, have this problem in common: they have been exposed to a plethora of foreign antigens and proteins. This is why people in the AIDS "risk groups" tend to have positive WBs (i.e., to be considered "HIV-infected") and people in the population don't. However, even people in low-risk populations have false-positive Western blots for poorly understood reasons 47.

Since false-positives to every single HIV protein have been documented 36, how do we know if the positive WB bands represent the various proteins to HIV, or a collection of false-positive bands reacting to several different non-HIV antibodies?"

sorry no

No question? Scarey?

Well, the RNA test does measure viral load.... so if you ahve HIV it will yeild a viral load thus you have HIV. But if the test comes back as undetectable then you most likly do not have it. But the most common form of HIV testing is an HIV antibody test. This test searches for HIV ANTIBODIES NOT THE VIRUS. However, it can take up to 3 months to form HIV antibodies after the initial infection. So basically if you had a high risk and can't wait for 3 months you should have a PCR-RNA test done which would look for the virus...these tests are usually 100% accurate after 1 month after the infection. If you have a negative PCR-RNA test you should re-test at the 3 month mark using a HIV antibody test. I don't know where you read this information from but there are hundreds of HIV test avaiable and all of them are intended for diagnosing HIV infection

what the hell do i care

Hi there,

Viral load testing is used with people already known to be infected with HIV. The most sensitive tests today still can only screen for the presence of the virus at least 50 copies per millimetre of blood. Any less and the test result states "undetectable". Simply being undetectable means that there is such a little amount of the virus in the blood that the tests can not pick it up but that does not mean the person is no longer infected.

Presently, the "gold standard" for HIV testing is the ELISA. If that test is negative, meaning that antibodies are not found, the testing is complete. If the ELISA test is positive, the laboratory will want to make sure that it is not a "false positive" result (some molecules in the bloodstream can sometimes cause a false-positive result). First, they may repeat the ELISA test. If it's positive, they will conduct a test called Western blot. If both the ELISA and the Western blot test yield a positive result, a diagnosis of HIV infection is confirmed and the results are sent back to the healthcare professional who ordered the test.

So it is the presence of antibodies that differentiates whether a person is HIV+ or HIV-. Viral load testing does not screen for antibodies, it only detects the amount of the virus present in the blood. And since it can only screen 50 copies or more, there is that grey zone of <50 copies of the virus per millimeter of blood: if the viral load is undetectable does that mean the person is in fact infected or not? Hence why antibody screening is a bit more accurate.

Viral load is used by doctors to help determine the next choice of action with regards to managing the disease. Doctors look at the trends in the viral load count as well as the trend in white blood cell count (T-4) to determine disease progression. If it is warrented, geneotype and or phenotype testing is used to determine which strands of medications the virus is resistant to and which medications are still available.


Yes there are.

Diagnostic tests and quantitative tests are different. You can't use a viral load test for diagnostic purposes because it's not sensitive enough for that purpose. You need either a qualitative RNA test (expensive and cumbersome) or an antibody test.

And yes, the manufacturers DO state that qualitative RNA tests and antibody tests are intended for use in diagnosisng HIV infection. Just not the viral load tests, which are for a different purpose.

[Edit] Contrary to some people's assertions, the FDA has approved a number of HIV diagnostic tests. You can find them and their approval dates on this FDA webpage:

No HIV test ever made has FDA approval for the purposes of diagnosing HIV infection. GO check the CDC website..

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